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Size

96 Tests

Price

885.00

Catalog

EKU06486

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Description

Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Receptor 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Receptor 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Test Principle

No significant cross-reactivity or interference between Parathyroid Hormone Receptor 1 and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Parathyroid Hormone Receptor 1 and analogues was observed.

Specificity

This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone Receptor 1

Target's alterntive name

PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor

Shelf life

Use Human Parathyroid Hormone Receptor 1 (PTHR1) ELISA Kit before 8 months

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Sample Type

Tissue homogenates, Cell lysates and other biological fluids

Application

For research use only. Not for diagnostic procedures.

Storage Temperature

Short term: 4°C; Long term: see manual.

Target Name

Parathyroid Hormone Receptor 1

Assay Type

Double-antibody Sandwich

Species Reactivity

Homo sapiens (Human)

Estimated Turnaround Time

6-11 Business days

Detection Range

0.312-20 ng/mL

Research Area

Endocrinology

Detection Method

Colorimetric

Sensitivity

0.127 ng/mL

Shipping Conditions

Ice packs

Assay Time

3 hours

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