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Back to: Our Providers / bioma / Canine Parathyroid Hormone (PTH) ELISA Kit, 96 well plate

Size

96T

Price

872.00

Catalog

EKU06481

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Description

Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.

Assay principle

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Parathyroid Hormone (PTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample.

ELISA's stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Precision of the test

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV

Warnings

The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage recommendation

-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

ELISA's specificity

This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH). No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.

ELISA's cross-reactivity

This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

QC

The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternate gene name

iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin

Notes

For research use only. Not for diagnostic procedures.

Research main area

Metabolic pathway;Endocrinology;Hormone metabolism;

Protocol

Please see ELISA's datasheet, otherwise contact us

Samples to be analyzed

Serum, plasma and other biological fluids.

Verified reactivity

Canis familiaris; Canine (Dog)

Alternate protein number

Please refer to SwissProt

Gene number

Please refer to GenBank

Protein number

Please refer to Uniprot

Gene name

Parathyroid Hormone

Estimated production time

7-11 business days

Detection limits

9.88-800 pg/mL

ELISA detection

Colorimetric

Assay class

Competitive

Sensitivity limit

3,52 pg/mL

Use for

8 months

Shipping requirements

Blue ice

Assay duration

2 hours

Verified applications

ELISA

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