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Size

10µg

Price

222.00

Catalog

PKSH032854-10µg

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Background

Parathyroid hormone is the most important endocrine regulator of calcium and phosphorus concentration in extracellular fluid. This hormone is secreted from cells of the parathyroid glands and finds its major target cells in bone and kidney. Another hormone, parathyroid hormone-related protein, binds to the same receptor as parathyroid hormone and has major effects on development. Like most other protein hormones, parathyroid hormone is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid hormone is secreted as a linear protein of 84 amino acids.

Description

Hormone releasing factors and releasing hormones are  signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Stability and Storage

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 monthshs

Activity

Measured by its ability to induce cAMP accumulation in MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is less than 0.1 ug/ml

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 5% Mannitol, pH 4.0.

Synonym

Parathyroid Hormone; PTH; Parathormone; Parathyrin

Endotoxin

<1.0 EU per µg as determined by LAL test.

Purity

>95% as determined by reducing SDS-PAGE.

Source

Recombinants or rec. proteins

Sequence

Ser32-Gln115

Group

recombinants

Mol Mass

9.0 kDa

AP Mol Mass

14 kDa

Expressed Host

E.coli

Accession

P01270

Fusion tag

NA

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