Th1 Response

1. Bellet D., Schlumberger M., Bidart J.M., Assicot M., Caillou B., Motte P. et al. (1983) Production and in vitro utilization of monoclonal antibodies to human thyroglobulin. J. Clin. Endocrinol. Metab. 56; 530-533. 2. Ormanns W. and Schäffer R. (1983) The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques. Histochemistry 78; 177-180. 3. Wilson N.W., Pambakian H., Richardson T.C., Stokoe M.R., Makin C.A., and Heydeman E. (1986) Epithelial markers in thyroid carcinoma: an immunoperoxidase study. Histopathology 10; 815-829. 4. De Micco C., Ruf J., Carayon P., Chrestian M.-A., Henry J.-F., and Toga M. (1987) Immunohistochemical study of thyroglobulin in thyroid carcinomas with monoclonal antibodies. Cancer 59; 471-476.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Protease pre-treatment is not required. However high temperature unmasking with Unmasking Fluid G (Art. No. DE007) may enhance staining. If peroxidase staining systems are used it should be taken into consideration that thyroid epithelia and colloid contain plenty of endogenous peroxidases.

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.

We recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Human, cross-reacting with cattle, goat, guinea pig, pig, sheep

Human Thyroglobulin, native and reduced form

Please contact us to check the availability

Purified human Thyroglobulin

Go to UniProt/SwissProt

liquid concentrate

Primary Antibody

Store at 2-8°C

Thyroid gland

Mus musculus

1:10 - 1:50

IHC(P)

RBU/01

Mouse

IgG1

N/A