NELF-A (negative elongation factor A), NELF-B, NELF-C, NELF-D and NELF-E are components of the NELF complex, which is a a structure that negatively regulates Pol II-dependent transcription elongation. Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF. DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites. DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT.
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Nasal embryonic LHRH factor; Nasal embryonic luteinizing hormone releasing hormone factor; Nasal embryonic luteinizing hormone-releasing hormone factor; Nelf; NELF_HUMAN.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
KLH conjugated synthetic peptide derived from human NELF
No modification has been applied to this antibody
This antibody reacts specifically with NELF
For facs or microscopy Alexa 1 conjugate.
Rabbit (Oryctolagus cuniculus)
Alexa Fluor,ALEXA FLUOR® 594
Conjugated Primary Antibody
Polyclonals and antibodies
NELF Polyclonal Antibody
Purified by Protein A.
ALEXA FLUOR® 594
Alexa conjugate 1
1ug per 1ul