1x96 well plate
ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. What’s more, ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
GENTAUR's human ADAMTS1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for ADAMTS1 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, F253-A734) and your samples are measured with a biotin conjugated polyclonal from goat specific for ADAMTS1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human ADAMTS1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Catalog number;Description;Quantity -;96-well plate precoated with anti- human ADAMTS1 antibodies;1 ST0000-10;Lyophilized recombinant human ADAMTS1 standard;10ng/tube×2 AR1107;Biotinylated anti- human ADAMTS1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Sample the amount of 100ul/well of the standard solution at 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human ADAMTS1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin) to the ELISA plate. Keep in mind that each human ADAMTS1 standard solution and each sample be measured in duplicate.
A disintegrin and metalloproteinase with thrombospondin motifs 1;A disintegrin like and metalloprotease with thrombospondin type 1 motif 1;A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 1;ADAM metallopeptidase with thrombospondin type 1 motif 1;ADAM TS 1;ADAM TS1;ADAM-TS 1;ADAM-TS1;ADAMTS 1;ADAMTS;ADAMTS-1;Adamts1;ATS1_HUMAN;C3 C5;KIAA1346;METH 1;METH-1;METH1
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);1208;2741;4137;1850;3035;4726 ELISA standard deviations 42.28;117.9;202.7;98.05;176.03;302.5 CV(%);3.5;4.3;4.9;5.3;5.8;6.4
Concentration(pg/ml) + 0 + 93.7 + 187.5 + 375 + 750 + 1500 + 3000 + 6000 O.D. + 0.026 + 0.096 + 0.160 + 0.278 + 0.513 + 0.920 + 1.599 + 2.476
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
For quantitative measurement of human ADAMTS1 in cell culture supernatant, serum and plasma heparin .
the ELISA does not cross react with any other proteins except from closely related species
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
A disintegrin and metalloproteinase with thrombospondin motifs 1
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
ADAM metallopeptidase with thrombospondin type 1 motif, 1
Natural and recombinant human ADAMTS1
93.8pg/ml to 6000pg/ml