Th1 Response

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat I-PTH . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat I-PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat I-PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat I-PTH. The concentration of Rat I-PTH in samples can be calculated by comparing the OD of the samples to the standard curve.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The Stop Solution is acidic. Do not allow to contact skin or eyes.

No significant cross-reactivity or interference was observed.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Use Rat I-PTH (Intact Parathormone) ELISA Kit before 6 months

The kit is manufactured at ISO 9001 certified facilities.

For research use only. Not for diagnostic procedures.

Short term: 4°C; Long term: see manual.

Serum, Plasma, Biological Fluids

Signal transduction

6-11 business days

Rattus norvegicus

15.63-1000 pg/mL

Colorimetric

9.38 pg/mL

Ice packs

4.5 hours

Sandwich

I-PTH

Rat

NA

NA

NA

NA