Th1 Response

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Monkey PTH . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Monkey PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Monkey PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Monkey PTH. The concentration of Monkey PTH in samples can be calculated by comparing the OD of the samples to the standard curve.

Rhesus Monkeys proteins are often measured by ELISA on serum or plasma since consensus epitopes with the human ELISA are used for producing the antibodies of these ELISA test kits. Often cDNA of monkeys is used as alternative to human cDNA as a model for drug development. Monkeys are generally considered to be intelligent, particularly Old World monkeys.

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Use Monkey I-PTH (intact Parathormone) ELISA Kit before 6 months

No significant cross-reactivity or interference was observed.

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

The kit is manufactured at ISO 9001 certified facilities.

For research use only. Not for diagnostic procedures.

Short term: 4°C; Long term: see manual.

Serum, Plasma, Biological Fluids

Signal transduction

6-11 business days

15.63-1000 pg/mL

Colorimetric

Monkey I-PTH

9.38 pg/mL

4.5 hours

Ice packs

Sandwich

Monkey

NA

NA

NA

NA