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Size
96 Tests
Price
670.00
Catalog
EKE60888
Quick and safe buy process
Test Principle
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PTH . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTH and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PTH. The concentration of Human PTH in samples can be calculated by comparing the OD of the samples to the standard curve.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Shelf life
Use Human I-PTH (intact Parathormone) ELISA Kit before 6 months
Cross Activity
No significant cross-reactivity or interference was observed.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality Systems
The kit is manufactured at ISO 9001 certified facilities.
Application
For research use only. Not for diagnostic procedures.
Storage Temperature
Short term: 4°C; Long term: see manual.
Sample Type
Serum, Plasma, Biological Fluids
Research Area
Signal transduction
Estimated Turnaround Time
6-11 business days
Detection Range
15.63-1000 pg/mL
Detection Method
Colorimetric
Sensitivity
9.38 pg/mL
Assay Time
4.5 hours
Shipping Conditions
Ice packs
Assay Type
Sandwich
Species Reactivity
Human
Target's alterntive name
I-PTH
Target Name
NA
Specificity
NA
Precision
NA
Stability
NA