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Size
96 Tests
Price
885.00
Catalog
EKU06486
Quick and safe buy process
Description
Hormone releasing factors and releasing hormones are signaling molecules produced by glands in multicellular organisms. The glands that secrete Luteinizing hormones LHRG and LH, FSH comprise the endocrine signaling system. The term growth hormone releasing hormone GHRH is sometimes extended to include chemicals produced by cells that affect the same cell (autocrine or intracrine signaling) or nearby cells (paracrine signaling). Human recombinant LHRG and GHRH are produced in E. coli or in yeast cells.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone Receptor 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone Receptor 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV
Test Principle
No significant cross-reactivity or interference between Parathyroid Hormone Receptor 1 and analogues was observed.
Cross Activity
No significant cross-reactivity or interference between Parathyroid Hormone Receptor 1 and analogues was observed.
Specificity
This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone Receptor 1
Target's alterntive name
PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor
Shelf life
Use Human Parathyroid Hormone Receptor 1 (PTHR1) ELISA Kit before 8 months
Quality Systems
The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
Precaution of Use
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Sample Type
Tissue homogenates, Cell lysates and other biological fluids
Application
For research use only. Not for diagnostic procedures.
Storage Temperature
Short term: 4°C; Long term: see manual.
Target Name
Parathyroid Hormone Receptor 1
Assay Type
Double-antibody Sandwich
Species Reactivity
Homo sapiens (Human)
Estimated Turnaround Time
6-11 Business days
Detection Range
0.312-20 ng/mL
Research Area
Endocrinology
Detection Method
Colorimetric
Sensitivity
0.127 ng/mL
Shipping Conditions
Ice packs
Assay Time
3 hours